Obtaining DNA sequence from the UCSC Database

Obtaining genomic data from the UCSC database.

• Open the UCSC browser and navigate to the "Dec. 2013 (GRCh38/hg38)" human assembly.

• Enter the position chr4:133,148,410-133,195,705 and click submit.

• The appropriate view is here.

• Click the View-->DNA link to enter the "Get DNA in Window" page

• Sequence range and formatting adjustments are available, accepting the defaults and clicking "Get DNA" will retrieve the genomic sequence that was present in the browser view.

• Go back to the "Get DNA in Window" page

• Click the “extended case/color options” button and configure the display so that known gene exons are in bold, red and upper case letters.

  • Set default case set to "Lower" with the radio button.

  • Check the boxes for "Toggle Case" and "Bold" and enter 255 in the "Red" box on the GENCODE v24 line.

  • Click submit and view the newly formatted results.