Workflows and Reports Tab

Analyze Single Experiment Workflow

  • The analyze single experiment workflow allows one experiment to be tested. Activate the an experiment with desired thresholds applied and select Analyze Single Experiment from the Workflows & Reports list.

  • Confirm Network object count and threshold settings and click Apply.

  • Results are returned by ontology, statistics are -log(pValue) (1.3 = 0.05)

  • Results can be examined by clicking on the name. See Legend for more information about maps and networks. Note: network objects included in filtered sets display expression results in image.

  • Different results link to different types of data.

    • Pathway maps are nice interactive maps

    • Process networks and network statistics produce editable network maps.

    • GO Processes produce lists that can be used to draw networks.

    • Diseases link to disease pages.

Enrichment Analysis Workflow

  • This workflow requires at least 2 active experiments. Activate all 3 ColonMet experiments and refresh the browser page to confirm.

  • Select the Enrichment Analysis link to launch the workflow. Ensure the correct experiments are activated and click Next.

  • Fold change, p-value signal directionality can be adjusted on the next page. NOTE: the number of Network objects that meet threshold criteria are indicated. Clicking Apply launches the analysis.

  • Results are returned by ontology, statistics are -log(pValue) (1.3 = 0.05)

  • Results can be examined by clicking on the name. See Legend for more information about maps and networks. Note: network objects included in filtered sets display expression results in image.

  • Different results link to different types of data.

    • Pathway maps are nice interactive maps

    • Process networks produce editable network maps.

    • GO Processes produce lists that can be used to draw networks.

    • Diseases link to disease pages.

Compare Experiments Workflow

  • This workflow requires at least 2 active experiments. Activate all 3 ColonMet experiments and refresh the browser page to confirm.

  • Select the Compare Experiments link to launch the workflow. Ensure the correct experiments are activated and click Next.

  • Fold change, p-value signal directionality can be adjusted on the next page. NOTE: the number of Network objects that meet threshold criteria are indicated. Clicking Apply launches the analysis.

  • Genes passing thresholds in each experiment are compared and grouped in 3 categories:

  1. Unique

  2. Similar - present in more than one list

  3. Common - present in all lists

  • Results are returned by ontology, statistics are -log(pValue) (1.3 = 0.05)

  • Results can be examined by clicking on the name. See Legend for more information about maps and networks. Note: network objects included in filtered sets display expression results in image.

  • Different results link to different types of data.

    • Pathway maps are nice interactive maps

    • Process networks and produces editable network maps.

    • GO Processes produce lists that can be used to draw networks.

    • Diseases link to disease pages.

  • Histograms can be sorted according to statistics

  1. Statistically significant

  2. Deferentially effected

  3. Similarity by

  4. Particular

  • Histogram are interactive, network statistics panel reports results.

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Massachusetts Institute of Technology